PERAN KULTUR DALAM PENELITIAN  Punica granatum MENAIKKAN KADAR GLUTATHION PEROXYDASE PADA KULTUR HUVECs YANG DIPAPAR PLASMA PRE EKLAMPSI (article review)

Suhrawardi Suhrawardi

Authors

Suhrawardi Suhrawardi Poltekkes Kemenkes Banjarmasin

Keywords:

Culture, Cell, HUVECS

Abstract

The HUVECs (Human Umbilical Vein Endothelial Cells) cell culture model was used in this study to investigate the effects of Punica granatum extract on Glutathione Peroxidase (GPx) levels under preeclampsy conditions. HUVECs endothelial cell culture is an appropriate choice because of its ability to replicate the conditions of endothelial dysfunction that occur in preeclampsia, which affects the blood vessels of pregnant women. The advantage of this in vitro model is its ability to isolate and manipulate specific factors involved in oxidative stress and vascular dysfunction without the variability derived from systemic factors in the human body. By using cell cultures of HUVECs, this study allowed researchers to monitor cell responses to preeclamptic plasma exposure and pomegranate extract treatment in a more controlled and scalable manner. The use of cell culture provides greater insight into the molecular mechanisms involved in endothelial cell damage in preeclampsia and the potential for natural material-based therapies. Although this study was limited to in vitro experiments, the results obtained demonstrate the importance of cell culture as a model that can accelerate the understanding and development of therapies for preeclampsia

References

Januarsih J, Fitria Jannatul Laili, Megawati M. PUNICA GRANATUM MENAIKKAN KADAR GLUTATHION PEROXYDASE PADA KULTUR HUVECs YANG DIPAPAR PLASMA PRE EKLAMPSI. JCI [Internet]. 2024 Oct. 25 [cited 2025 Apr. 21];4(2):85-90. Available from: https://bajangjournal.com/index.php/JCI/article/view/8769

Roberts JM, Gammill HS. Preeclampsia: recent insights. Hypertension. 2005;46(6):1243–1249.

Redman CW, Sargent IL. Placental stress and pre-eclampsia: a revised view. Placenta. 2009;30 Suppl A:S38–S42.

Goulopoulou S, Davidge ST. Molecular mechanisms of maternal vascular dysfunction in preeclampsia. Trends Mol Med. 2015;21(2):88–97.

Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. J Clin Invest. 1973;52(11):2745–2756.

Aird WC. Phenotypic heterogeneity of the endothelium: I. Structure, function, and mechanisms. Circ Res. 2007;100(2):158–173.

Myatt L, Webster RP. Vascular biology of preeclampsia. J Thromb Haemost. 2009;7(3):375–384.

Noris M, Perico N, Remuzzi G. Mechanisms of disease: preeclampsia. Nat Clin Pract Nephrol. 2005;1(2):98–114.

Seeram NP, Aviram M, Zhang Y, Henning SM, Feng L, Dreher M, et al. Comparison of antioxidant potency of commonly consumed polyphenol-rich beverages in the United States. J Agric Food Chem. 2008;56(4):1415–1422.

Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kaplan M, Coleman R, et al. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation. Am J Clin Nutr. 2000;71(5):1062–1076.

Gil MI, Tomás-Barberán FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agric Food Chem. 2000;48(10):4581–4589.

Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines. Braz J Med Biol Res. 2000;33(2):179–189.

Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006;160(1):1–40.

Burton GJ, Redman CW, Roberts JM, Moffett A. Pre-eclampsia: pathophysiology and clinical implications. BMJ. 2019;366:l2381.

Wu J, Xia S, Kalionis B, Wan W, Sun T. The role of oxidative stress and inflammation in cardiovascular aging. Biomed Res Int. 2014;2014:615312.

Kwon JY, Lee YJ, Kim YJ, Lee KA. Relationship between oxidative stress and endothelial dysfunction in pregnant women with preeclampsia. Am J Obstet Gynecol. 2011;204(5):364.e1–e6.

Poston L, Raijmakers MTM. Trophoblast oxidative stress, antioxidants and pregnancy outcome—a review. Placenta. 2004;25:S72–S78.

Al-Kuraishy HM, Al-Gareeb AI. Pomegranate and its pharmacological importance: a review. J Pharmacogn Phytother. 2016;8(2):39–46.

Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010;2(12):1231–1246.

Aghaei M, Sadeghnia HR, Asadpour E, Boroushaki MT, Azarmi Y, Mashkani B. Protective effect of pomegranate seed oil against oxidative stress induced by tert-butyl hydroperoxide in HUVECs. Avicenna J Phytomed. 2015;5(5):404–412.