OPTIMIZATION OF SOLVENT AND CONCENTRATION OF TURMERIC (Curcuma longa Linn.) EXTRACT FOR STRIP-TEST AS BORAX DETECTION TOOL
DOI:
https://doi.org/10.53625/ijss.v1i6.1922Keywords:
Turmeric, Borax, Borax Detector, Qualitative Analysis, Concentration OptimizationAbstract
Borax, a well-known toxic substance, had been banned as food additive in many countries, including Indonesia. Vomiting, fatigue, renal failure, cancer and even sudden death were reported as its toxic effect. There are many studies about Borax identification had been carried out, including instrumental procedures and alternative tests using natural indicator from the plants, such as turmeric. Alternative tests were low-cost, simple and easy to do. However, a certain solvent and concentration of turmeric for borax detection has not studied yet, which made a gap in borax analysis using a natural indicator. This research aims to determine the effect of turmeric solvent extraction and its concentration on borax detection results using the Turmeric Paper Test Kit. Turmeric powder was macerated with three different solvents (methanol, ethyl acetate and n-hexane) for 3 days. The highest yielded extract was methanol extract, followed by ethyl acetate and n-hexane extract. Solution of Turmeric extract in various solvents were prepared in many concentrations and dripped on paper of strip-test detection tool and leaved to dry. Borax detection was performed with dripped on strip-test detection tools, and discoloration of turmeric paper was observed. The most optimal solvent in this research is methanol extract with 10.000 ppm concentration, and discoloration to reddish-brown color
References
Kementerian Kesehatan RI, “PerMenKes Republik Indonesia No. 33 tentang Bahan Tambahan Pangan,” PerMenKes Republik Indones. No. 33, pp. 1–10, 2012.
BPOM, “Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 36 Tahun 2013 Tentang Batas Maksimum Penggunaan Bahan Tambahan Pangan Pengawet,” pp. 1–16, 2013.
Sugiatmi and I. A. Poetra, “Knowledge and Practice of the Use of Borax in Meatballs Seller At the Campus a University of Muhammadiyah Jakarta,” in Proceedings:The 2nd International Multidisciplinary Conference, 2016, vol. 1, no. 1, pp. 744–750.
C. Dinh Viet et al., “Simultaneous determination of borax and polyphosphates content in food by liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS),” Heavy Met. Arsen. Conc. water, Agric. soil, rice Ngan Son Dist. Bac Kan Prov. Vietnam, vol. 4, no. 2, pp. 115–126, 2021, doi: 10.47866/2615-9252/vjfc.3788.
L. Jing et al., “A novel borax-specific ssDNA aptamer screened by high-throughput SELEX and its colorimetric assay with aggregation of AuNPs,” J. Food Compos. Anal., vol. 101, no. November 2020, p. 103947, 2021, doi: 10.1016/j.jfca.2021.103947.
I. P. Sari, F. A. Yanti, D. I. Saefullah, and B. T. Yunianto, “Identification of borax in meatballs at Ciroyom Market, Bandung City, Indonesia,” J. Sustain. Sci. Technol., vol. 1, no. 1, pp. 44–51, 2021, doi: 10.23960/josst.v1i1.8.
Aseptianova, D. Afriansyah, and M. Astriani, “Penyuluhan Bahan Makanan Yang Mengandung Boraks Di Kelurahan Kebun Bunga Kota Palembang,” J. Batoboh, vol. 2, no. 1, pp. 5–24, 2017.
M. Kabu, M. Tosun, B. Elitok, and M. S. Akosman, “Evaluación histológica de los efectos del borax obtenido de diversas fuentes en diferentes Órganos de rata,” Int. J. Morphol., vol. 33, no. 1, pp. 255–261, 2015, doi: 10.4067/S0717-95022015000100040.
A. W. Prasetya and L. Dewi, “Deteksi Kandungan Rhodamin B Pada Saus Serta Cemaran Boraks Dan Bakteri Salmonella Sp. Padacilok Keliling Salatiga,” Agric, vol. 28, no. 1, p. 69, 2017, doi: 10.24246/agric.2016.v28.i1.p69-78.
L. K. Muharrami, “Analisis kualitatif kandungan Boraks pada krupuk puli di Kecamatan Kamal,” J. Pena Sains, vol. 2, no. 2, 2015.
Erniati, “Tingkat Pendidikan, Pengetahuan, Sikap Pedagang Bakso dan Penggunaan Boraks Pada Bakso di SDN Lemahputro III Sidoarjo,” J. Kesehat. Lingkung., vol. 9, no. 2, pp. 209–216, 2017.
D. Suseno, “Analisis Kualitatif dan Kuantitatif Kandungan Boraks Pada Bakso Menggunakan Kertas Turmerik, FT – IR Spektrometer dan Spektrofotometer Uv -Vis,” Indones. J. Halal, vol. 2, no. 1, p. 1, 2019, doi: 10.14710/halal.v2i1.4968.
Nurkholidah, M. Ilza, and C. Jose, “Analisis Kandungan Boraks Pada Jajanan Bakso Tusuk Di Sekolah Dasar Di Kecamatan Bangkinang Kabupaten Kampar,” J. Ilmu Lingkung., vol. 6, no. 2, pp. 134–145, 2013, [Online]. Available: http://ejournal.unri.ac.id/index.php/JIL/article/view/963.
C. Rahma and Y. Hidjrawan, “Qualitative Identification of Borax Content in Meatball Snacks Using Turmeric Paper and Shallot Extract,” J. Pangan dan Agroindustri, vol. 9, no. 1, pp. 56–63, 2021, doi: 10.21776/ub.jpa.2021.009.01.6.
N. Ratnarathorn and W. Dungchai, “Paper-based Analytical Device (PAD) for the Determination of Borax, Salicylic Acid, Nitrite, and Nitrate by Colorimetric Methods,” J. Anal. Chem., vol. 75, no. 4, pp. 487–494, 2020, doi: 10.1134/S1061934820040127.
R. E. Y. Adu, R. Roto, and A. Kuncaka, “Spectrophotometric Determination of Boron in Food Products By Ester Borate Distillation Into Curcumin,” vol. 15, no. 1, pp. 67–73, 2021.
Q. A. Lathifah, L. Turista, Dora Dayu Rahma Azizah, and A. E. Khulaifi, “Identification of formalin and borax on tuna in Ngemplak market Tulungagung regency,” Med. Lab. Anal. Sci. J., vol. 1, no. 1, pp. 1–5, 2019.
T. Septiani and A. P. Roswien, “Analisis Kualitatif Kandungan Boraks Pada Bahan Pangan Daging Olahan dan Identifikasi Sumber Boron dengan FTIR – ATR,” Indones. J. Halal, vol. 1, no. 1, p. 48, 2018, doi: 10.14710/halal.v1i1.3403.
M. Yunianto, D. P. Andaresta, F. Anwar, and T. E. Saraswati, “Preliminary study on the use of optical fiber to detect borax content in meat,” J. Phys. Conf. Ser., vol. 1825, no. 1, 2021, doi: 10.1088/1742-6596/1825/1/012078.
N. Rahman, “Formalin and Borax Qualitative Test Use Natural Indicator,” J. Phys. Conf. Ser., vol. 1363, no. 1, 2019, doi: 10.1088/1742-6596/1363/1/012092.
E. D. Astuti and W. S. Nugroho, “Kemampuan Reagen Curcumax Mendeteksi Boraks dalam Bakso yang Direbus,” J. Sain Vet., vol. 35, no. 1, p. 42, 2017, doi: 10.22146/jsv.29289.
R. Samson, “BoDex : A User-friendly Qualitative Test Kit,” pp. 1–18, 2015.
S. Harimurti, I. H. Bariroh, A. Setiyawan, R. I. Permatasari, F. D. Putri, and L. Y. Fajriana, “Identification of the Spread of Borax Use in Meatball Skewers in Bantul District, Special Region of Yogyakarta,” J. Pharm. Sci. Community, vol. 17, no. 2, pp. 120–130, 2020, doi: 10.24071/jpsc.002369.
H. Rahman, M. Prajuwita, D. Z. Yanni, P. M. Sari, and I. Lestari, “Analysis of Borax in Ground Red Chilies in the Traditional Markets of Jambi City,” EduChemia (Jurnal Kim. dan Pendidikan), vol. 5, no. 1, p. 10, 2020, doi: 10.30870/educhemia.v5i1.6520.
T. E. Purbaningtias, I. D. Lestari, B. Wiyantoko, P. Kurniawati, and D. Sriadryani, “Utilization of natural indicators for borax identification in the Indonesian tofu,” AIP Conf. Proc., vol. 1823, no. March 2017, 2017, doi: 10.1063/1.4978130.
T. Prathumsuwan, P. Jaiyong, I. In, and P. Paoprasert, “Label-free carbon dots from water hyacinth leaves as a highly fluorescent probe for selective and sensitive detection of borax,” Sensors Actuators, B Chem., vol. 299, no. July, p. 126936, 2019, doi: 10.1016/j.snb.2019.126936.
G. Grynkiewicz and P. Ślifirski, “Curcumin and curcuminoids in quest for medicinal status,” Acta Biochim. Pol., vol. 59, no. 2, pp. 201–212, 2012, doi: 10.18388/abp.2012_2139.
K. Doldolova, M. Bener, M. Lalikoğlu, Y. S. Aşçı, R. Arat, and R. Apak, “Optimization and modeling of microwave-assisted extraction of curcumin and antioxidant compounds from turmeric by using natural deep eutectic solvents,” Food Chem., vol. 353, no. January, 2021, doi: 10.1016/j.foodchem.2021.129337.
S. Tripathy et al., “Curcumin Extraction, Isolation, Quantification and Its Application in Functional Foods: A Review With a Focus on Immune Enhancement Activities and COVID-19,” Front. Nutr., vol. 8, no. September, 2021, doi: 10.3389/fnut.2021.747956.
M. Valizadeh Kiamahalleh, G. Najafpour-Darzi, M. Rahimnejad, A. A. Moghadamnia, and M. Valizadeh Kiamahalleh, “High performance curcumin subcritical water extraction from turmeric (Curcuma longa L.),” J. Chromatogr. B Anal. Technol. Biomed. Life Sci., vol. 1022, pp. 191–198, 2016, doi: 10.1016/j.jchromb.2016.04.021.
K. I. Priyadarsini, “The Chemistry of Curcumin: From Extraction to Therapeutic Agent,” Molecules, vol. 19, pp. 20091–20112, 2014, doi: 10.3390/molecules191220091.
A. Rohman, “Analysis of curcuminoids in food and pharmaceutical products,” in International Food Research Journal, 2012, vol. 19, no. 1, pp. 19–27.
